gene regulation , transcription factor , DNA binding , cell sorting , DNA sequencing

Mapping DNA sequence to transcription factor binding energy in vivo

Update: This paper has now been published in PLOS Comp Bio. “Mapping DNA sequence to transcription factor binding energy in vivo” by Stephanie L Barnes, Nathan M Belliveau, William T Ireland, Justin B Kinney and Robert Phillips https://doi.org/10.1101/331124 In this article, Barnes and colleagues constructed a libraries of strains in which GFP expression is controlled by a transcription factor, and the transcription factor (TF) binding sites were randomly mutated. Libraries were sorted by GFP fluorescence and sequenced to determine the likelihood that a given binding site corresponds to a range of fluroescence. »
Zach Hensel Author image
gene regulation , noise , stochastic modeling , fluorescence microscopy , single cell , microbiology , antibiotic resistance , transcription factor

Active degradation of a regulator controls coordination of downstream genes

This paper has now been published at PLOS Computational Biology. “Active degradation of a regulator controls coordination of downstream genes” by Nicholas A. Rossi, Thierry Mora, Aleksandra M. Walczak, and Mary J. Dunlop https://doi.org/10.1101/272120 In this article, Rossi and colleagues observed how noise develops in the expression of genes downstream of stress response activator gene MarA by manipulating MarA degradation and expression levels and then measuring variability in downstream gene expression in single cells. »
Zach Hensel Author image